Experiment : Plasmolysis

Study of Plant carrels in Hypertonic Solution
Objective
To observe the cells of an onion peel in hypertonic solution.
Apparatus and material required
An onion, film over slide, coverslip, forceps, needle, mop, blade, blotting paper, dropper,concentrated brininess or sugar solution and a increase microscope.
Theory
When a plant cell is put in a s solution that has a higher solute concentration(hypertoniccompared to the cell sap) piss is lost from the cell by osmosis (exosmosis). As theconcentration of water molecules in the cell is higher than the step forwarder hypertonic solution, netmovement of water molecules is from their piece of higher concentration to their region of lower concentration across the selectively permeable plasma membrane. This process iscalled exosmosis. If exosmosis continues in a plant cell, cytoplasm along with the nucleusand vacuole shrinks to a small strong mass payable to loss of water. Eventually it pulls a trendform the cell wall. The berth between, the cell wall and cytoplasmic mass is filled with thesalt or sugar solution due to the permeability of the cell wall. This phenomenon isplasmolysis and the cell is said to be plasmolysed.Plasmolysis is a reversible phenomenon.



When the plasmolysed cell is placed in water,which is hypotonic compared to cell sap, the cell gains water due to endosmosis. As a result. The cell membrane, cytoplasm and vacuole regain their traffic pattern position. This phenomenonis called deplasmolysis.
Procedure
1.Remove the outermost peel of the onion.2.Cut out a small bit from an inner fleshy scale leaf using the blade.3.Pull out the transparent peel of the scale leaf using the forceps.4.Using the brush place the piece of onion peel on a clean glass slide. Put a drop of theconcentrated salt or sugar Solution on the peel with the overhaul of the dropper.5.Put a clean coverslip by tilting it over the needle. In this way you can prevent airbubbles form getting trapped chthonian the coverslip.6.Remove excessive solution with the blotting...If you want to get a in force(p) essay, order it on our website: Orderessay



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